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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Pediatr Surg. 2013 Jun;48(6):1198–1204. doi: 10.1016/j.jpedsurg.2013.03.013

Figure 3.

Figure 3

Whole mount lung bud staining at E14.5 at 5X magnification. Lymphatic endothelial cells were localized with anti-LYVE-1-Cy3 (red). Endothelial cells were localized with anti-CD31-FITC (green). Normal lung buds (A and B) have linear, organized lymphatic endothelial cells with homogeneous distribution throughout the lung bud. N/B+CDH lung buds (C and D) are hypoplastic, and lymphatic endothelial cells are disorganized, nonlinear, with heterogeneous distribution throughout the lung bud. N/B-CDH lung buds (E and F) have left lung hypoplasia, but less severe than that seen in N/B+CDH. Lymphatics in N/B-CDH lung buds are more organized and linear than in N/B+CDH lung buds.