FIG. 6.

Decreased endogenous LMP-1 mRNA and protein levels in M-phase-arrested cells. (A) RPA was performed on total RNA from asynchronous (Asy) and M-phase-arrested X50-7 cells (M) with GAPDH and LMP-1 probes (left panel) or GAPDH and EBNA-2 probe (right panel). Yeast RNA and total RNA from DG75 cells were used as negative controls. A representative result from three independent experiments is shown. (B) Relative mRNA levels of LMP-1 and EBNA-2 were analyzed by normalizing LMP-1 (left panel) or EBNA-2 mRNA (right panel) levels to the GAPDH mRNA level with a PhosphorImager. Each data point represents the average of three independent experiments. Error bars represent the means ± standard errors of the means. *, P < 0.01. (C) Western blot of LMP-1 from total lysates of asynchronous (Asy) and M-phase-arrested X50-7 cells (M). β-actin levels were used as a loading control.