Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2004 Apr;78(7):3470–3479. doi: 10.1128/JVI.78.7.3470-3479.2004

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2004, American Society for Microbiology

PMC Copyright notice

FIG. 6. — Infection assay. To test whether the spacers increase the infection efficiency, hAd5CMV-GFPΔpIX viruses were propagated in 911 cells expressing the pIX.RGD variants. The capacity of the resulting viruses to transduce cells independent of CAR was tested by exposing CAR-negative Eoma cells to the CsCl purified viruses at 1,000 particles/cell. At 48 h postinfection, the cells were evaluated for GFP expression. (A to F) hAd5CMV-GFPΔpIX with wt.pIX (A), pIX.RGD (B), pIX.flag.RGD (C), pIX.flag.30.RGD (D), pIX.flag.45.RGD (E), or pIX.flag.75.RGD (F). To show that hAd5CMV-GFPΔpIX with wt.pIX (G) and hAd5CMV-GFPΔpIX bearing pIX.flag.75.RGD (H) have equivalent infectious particle numbers, HeLa cells were infected with the corresponding viruses.