TABLE 1.
Primer, orientationa | Sequence (5′-3′)b |
---|---|
protIX, For | CCGGAATTCGCCATGAGCACCAACTCGTTT |
protIX, Rev | TGCTCTAGATTAGCCGTCGGCCAGCTTGTAGTCGCTCCCTCCGCCGTTAACCGCATTGGGAGG |
Flag XE,For | AACGGCGGAGGGAGCGACTACAAGCTGGCCGACGGCCTCGAGGATATCTAGT |
Flag XE,Rev | CTAGACTAGATATCCTCGAGGCCGTCGGCCAGCTTGTAGTCGCTCCCTCCGCCGTT |
pIXstop, For | CCTCCCCTCCCAATGCGGTTTAAGGCGGAGGGAGCGACTACAA |
pIXstop, Rev | TTGTAGTCGCTCCCTCCGCCTTAAACCGCATTGGGAGGGGAGG |
RGD, For | TGTGATTGTAGAGGCGATTGCTTCTGCGGATGA |
RGD, Rev | CTAGATCATCCGCAGAAGCAATCGCCTCTACAATCACAGGC |
30 spacer, For | TCGAGATCCCAACCTATCTGAGCGAAGATGAACTGAAAGCCGCCGAAGCCGCCTTCAAACGCCACAACCCAACCGCGG |
30 spacer, Rev | TCGACCGCGGTTGGGTTGTGGCGTTTGAAGGCGGCTTCGGCGGCTTTCAGTTCATCTTCGCTCAGATAGGTTGGGATC |
ApoE, For | ACCGGTCGACGAGGAGACGCGGGCACGGCT |
ApoE, Rev | ATCCTCGAGGGCATCCTCGCCGCGGTACTGC |
MYCa, For | GGCGCCGAGCAGAAACTCATCTCTGAAGAAGATCTGGAACAAAAGTTGATTTCAGAA |
MYCa, Rev | CTTCTTCTGAAATCAACTTTTGTTCCAGATCTTCTTCAGAGATGAGTTTCTGCTCGGCGCC |
MYCb, For | GAAGATCTGGAACAGAAGCTCATCTCTGAGGAAGATCTGCACGTGTAAGGGCC |
MYCb, Rev | CTTACACGTGCAGATCTTCCTCAGAGATGAGCTTCTGTTCCAGAT |
pIXScaI, For | GTTTTGCAGCAGCCGCCGCCAGTACTAGCACCAACTCGTTTGATGG |
pIXScaI, Rev | CCATCAAACGAGTTGGTGCTAGTACTGGCGGCGGCTGCTGCAAAAC |
pIXSpeI, For | GGTTTCTGCCCTGAAGGCTTACTAGTCTCCCAATGCGGTTTAAAAC |
pIXSpeI, Rev | GTTTTAAACCGCATTGGGAGACTAGTAAGCCTTCAGGGCAGAAACC |
For, forward; Rev, reverse.
Restriction sites mentioned in Materials and Methods are underscored.