Figure 1.

NLK interacts with ATXN1. A, Coaffinity purification (Co-AP) assay shows that ATXN1(30Q) interacts with both WT and kinase activity mutant (KN) NLK. KN is a lysine to methionine substitution at residue 155 (K155M). Co-AP assay was performed in HEK293T cells. Top, The presence of Flag-NLK after affinity purification on glutathione-Sepharose 4B beads, demonstrating the ATXN1-NLK interaction. GST-empty vector (−) was used as a control. GFP expression was used as a transfection and loading control. B, The N-terminal region (amino acids 1–575) of ATXN1 interacted most strongly with NLK. Bottom, Schematic representation of the ATXN1 constructs.