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. 2013 May 29;33(22):9328–9336. doi: 10.1523/JNEUROSCI.3465-12.2013

Figure 6.

Figure 6.

Expression levels and ATXN1-associated protein complex formation in Atxn1154Q/+; Nlk+/− mice. A, Nlk heterozygosity does not affect the expression levels of components in Atxn1 native complexes in cerebellar extracts of SCA1 knock-in mice. Expression of Capicua (CIC-L and CIC-S, long and short isoforms of CIC, respectively), WT (Atxn1[2Q]), and polyglutamine-expanded mutant (Atxn1[154Q]) Atxn1 and Rbm17 remained the same in cerebellar extracts from 20-week-old Atxn1154Q/+ or Atxn1154Q/+; Nlk+/− mice. Nlk and Gapdh were used as controls. B, The level of NLK incorporation in toxic large complexes is low in Atxn1154Q/+; Nlk+/− mice compared with Atxn1154Q/+ mice. Elution profiles of CIC, Atxn1 (both WT and mutant Atxn1), Rbm17, and Nlk proteins in Atxn1154Q/+ or Atxn1154Q/+; Nlk+/− mouse cerebellum using gel-filtration chromatography. Representative Western blots of 1.0 ml gel-filtration fractions of Atxn1154Q/+ or Atxn1154Q/+; Nlk+/− mouse cerebellar extracts analyzed for CIC, Atxn1, Rbm17, and Nlk. The column void volume (Vo), gel-filtration standards thyroglobulin (669 kDa) and ADH (150 kDa), and elution volume (ml) of each collected fraction are indicated. Ex, Extract.