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. 2013 Jul 8;13:336. doi: 10.1186/1471-2407-13-336

Figure 1.

Figure 1

CysLT2R expression in ATRA-treated colon cancer cell lines. Cells were incubated for 3, 12, or 24 h in the absence or presence of 1 μM ATRA as indicated. (A and C) qPCR for CysLT2R expression. Data are normalized to HPRT-1 in SW480 and Caco-2 cells. (B and D) Protein expression of CysLT2R normalized to β-actin in SW480 and Caco-2 cells as determined by Western blot. (E) qPCR for CysLT1R expression in SW480 cells. Data are normalized to HPRT-1. (F) Protein expression of CysLT1R normalized to β-actin in SW480 cells as determined by Western blot. Changes in CysLT2R expression relative to basal levels in unstimulated cells are shown as mean ± standard error of the mean (SEM; *P < 0.05, **P < 0.01; n.s. = not significant).