Figure 3.

Effect of ATRA on cell growth, apoptosis, LTC₄S and LTC₄ expression. SW480 cells were treated with the CysLT₂R inhibitor AP 100984 (AP) and/or 40 nM LTC₄ and were or were not stimulated with 1 μM ATRA. (A) ³H-thymidine incorporation measured after 72 h of ATRA treatment, normalized to unstimulated control. (B) Caspase-3 activity after 48 h of ATRA treatment normalized to unstimulated control. (C) qPCR for LTC₄S expression after 3–24 h of ATRA stimulation. Data are normalized to HPRT-1. (D) Release of LTC₄ into the media form SW480 cells after 24 h incubation in the absence or presence of ATRA. Values are shown as mean ± SEM (*P < 0.05, **P < 0.01).