FIG. 2.
The FMDV Lbpro induces cleavage of eIF4GI and eIF4GII within cells. Human 293 cells were infected with the recombinant vaccinia virus vTF7-3 and transfected with the indicated plasmids that express FMDV Lbpro (pLb) or the PV 2Apro (pAΔ802) with Lipofectin. After 20 h, cell extracts were prepared and analyzed by SDS-PAGE and immunoblotting with antisera specific for the N termini of eIF4GI (A) and eIF4GII (B). The cleavage products and the full-length proteins are indicated. An additional cleavage product detected in extracts containing the FMDV Lbpro is highlighted by an asterisk.