FIG. 3.
Cleavage of eIF4GI and eIF4GII by the FMDV Lbpro in cell extracts is enhanced by the presence of added eIF4E. Extracts of uninfected human 293 cells (100 μg of protein) were incubated with the indicated amounts of recombinant FMDV Lbpro or CVB4 2Apro (7 μg) in the presence (+) or absence (−) of recombinant eIF4E (1 μg) as indicated. Samples were analyzed by SDS-PAGE (10% polyacrylamide) and immunoblotting with antibodies specific for the C terminus of eIF4GI (A) or eIF4GII (B). Proteins were detected using chemiluminescence reagents as in Fig. 1.