FIG. 5.
Comparison of in vitro cleavage sites in eIF4GI and eIF4GII by FMDV Lbpro and enterovirus 2Apro. An alignment of a central region of human eIF4GI and eIF4GII including the eIF4E binding sites together with the regions of the proteins cleaved by FMDV Lbpro and enterovirus-rhinovirus 2Apro is shown. The positions of the cleavage sites determined from in vitro analyses are indicated within the boxes. The five amino acid residues, determined by N-terminal sequence analysis, of the C-terminal cleavage product obtained from the GST-eIF4GII-FLAG fusion protein following incubation with recombinant FMDV Lbpro are underlined with a dashed bar.