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Deletion of the transmembrane domain of HCV NS5B abolishes replication of subgenomic replicons in Huh-7 cells. In vitro-transcribed replicons were transfected into Huh-7 cells, and G418-resistant colonies were stained with 0.2% crystal violet 3 weeks after transfection. For Rep/GDD, the GDD catalytic motif of HCV NS5B was changed to AAA.
