FIG. 4.

The C-terminal transmembrane peptide affects the membrane association of HCV NS5B in Huh-7/replicon cells. Cells were treated with the indicated peptide at 25 μM or with DMSO alone for 48 h. Postnuclear lysates were analyzed by a membrane flotation assay. Fractions were collected from the top to the bottom of the gradient, and each fraction was analyzed for HCV NS5B (A) or calnexin (B) by immunoblotting. Experiments were repeated three times, and the results of representative experiments are shown.