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. 2013 Jul 1;11:59. doi: 10.1186/1477-7827-11-59

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Copyright © 2013 Pujianto et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blots analyses confirmed the presence of SPAG11A protein in the epididymis. Fifteen micrograms of protein from each region of the epididymis was separated by 10% SDS-PAGE and transferred to PVDF membranes. SPAG11A was detected with a rabbit anti-human SPAG11A antibody. IS = initial segment; Cap = Caput; Cor = Corpus; Cau = Cauda. The results showed the presence of SPAG11A (MW = 20 kDa, indicated by an arrow) in the mouse caput epididymis. A very weak and smear band of SPAG11A was detected in the corpus, whereas no signals were observed in the initial segment and cauda. The same membrane was stripped and re-probed with antibody against α-tubulin as a loading control.