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Immunohistochemistry to confirm the dependence of SPAG11A protein expression on androgen. Staining using SPAG11A polyclonal antibody was performed on caput epididymal tissue sections from a normal non castrated mouse and 3 d castration. The results showed consistent staining in the nucleus and faint staining in the cytoplasm of the caput principal cells in the non castrated mice and the staining disappeared after 3 d castration. The images were observed at 400× magnification.
