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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Cancer Discov. 2013 Apr 4;3(7):782–797. doi: 10.1158/2159-8290.CD-12-0215

Figure 3. DGKα inhibition and phosphatidic acid synthesis.

Figure 3

A, U87 and B, U251 GBM cells, and C, A-375 melanoma cells were transfected with control or DGKα siRNA with simultaneous administration of exogenous PA at 33 µM or vehicle (1 MeOH : 2 Chloroform, v:v). Full phenotypic rescue of decreased cell viability was observed upon delivery of PA to DGKα siRNA transfected cells. Phenotypic rescue was achieved in D, U87 and E, U251 GBM cells treated with R59022 at 5 µM. F, After transfection with siRNA, mass spectrometry was utilized to show a decrease in total PA levels in lipid lysates from U251 cells. (*, P<0.05 and **, P<0.01 Student t test)