Figure 4.
ATMR-NLS demonstrates enhanced sensitivity compared to the ATMR. (a) Treatment of 293 cells bearing either a control luciferase (Luc), ATMR, ATMR-NLS, or ATMR-NLSmut for one hour with CGK-733 (10 μM), followed by assaying for bioluminescence. (b) 293 cells bearing various reporters were irradiated with 2 Gy and bioluminescent activity was monitored at 5, 10, 15, 30, 60, and 120 min after radiation. Data are normalized to values from 293-Luc cells, asterisks indicate p-value < 0.05 compared to 293-Luc cells, and § indicates p-value < 0.05 compared to 293-ATMR cells. Error bars are s.e.m.