FIG. 4.

Reduction of IgG2a/IgG1 ratios by rIL-4 treatment in orally infected mice. BALB/c mice were infected either orally or in the FP, and some orally infected mice received rIL-4 at 3 μg/dose (with or without 30 μg of anti-IL-4 MAb carrier/dose as described in the text) on day −1 and on day 2 of infection. The serum IgG subclass responses were determined by ELISA 10 days following infection. Data are represented as the ratio of the geometric means of IgG2a and IgG1 titers. Bars indicate the standard errors among groups of 5 to 11 mice from two independent experiments. Asterisks indicate significant differences from orally infected mice as determined by a one-way ANOVA and Dunn's post-hoc test (P < 0.05).