TABLE 3.
Alignment of amino acids in drug-binding pocket of HRV-B virusesa
| Serotype | EC50 (μM)b | Amino acid at position:
|
||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 104 | 105 | 106 | 107 | 124 | 126 | 128 | 130 | 150 | 151 | 152 | 174 | 175 | 176 | 186 | 188 | 191 | 197 | 199 | 215 | 219 | 221 | 224 | 245 | 267 | ||
| Majority sequence | I | N | L | S | F | S | Y | I | A | M | Y | P | S | V | F | V | V | Y | C | I | N | M | M | H | G | |
| Serotype | ||||||||||||||||||||||||||
| 83 | 0.02 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 92 | 0.02 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 35 | 0.04 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 79 | 0.04 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 6 | 0.06 | - | - | F | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | I | - | - |
| 37 | 0.06 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 3 | 0.09 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 86 | 0.13 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | I | - | - |
| 14 | 0.16 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 17 | 0.23 | - | S | - | - | - | - | - | - | - | - | - | - | - | - | - | - | I | - | - | - | - | - | I | - | - |
| 70 | 0.30 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | I | - | - |
| 91 | 0.73 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | I | - | - | - | - | - | I | - | - |
| 72 | 0.94 | - | S | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| 26 | 1.60 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | L | - | - | - | - | - | - | - | - |
| 48 | 1.80 | - | S | - | - | - | - | - | - | - | - | - | - | - | - | - | - | T | - | - | - | - | - | I | - | - |
| 52 | 2.49 | V | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | I | - | - |
| 27 | 5.90 | - | - | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | N | - | - | - | I | - | - |
| 69 | 6.68 | - | S | - | - | - | - | - | - | - | - | - | - | - | - | - | - | T | - | - | - | - | - | I | - | - |
| 4 | >12.5 | - | S | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | - | - | - | - | I | - | - |
| 5 | >12.5 | - | - | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | - | - | - | - | - | - | - |
| 42 | >12.5 | - | - | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | - | - | - | - | - | - | - |
| 84 | >12.5 | - | - | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | N | - | - | - | - | - | - |
| 93 | >12.5 | - | - | - | - | - | - | - | - | - | - | F | - | - | I | - | - | L | - | N | - | - | - | I | - | - |
| 97 | >12.5 | - | - | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | N | - | - | - | - | - | - |
| 99 | >12.5 | - | - | - | - | - | - | - | - | - | - | F | - | - | - | - | - | L | - | - | - | - | - | I | - | - |
a
Amino acid residues in the drug-binding pocket are defined as those for which any atom is within 4 Å of compounds bound in HRV-14 (27). Amino acid numbering is based on VP1 sequence of HRV-14 (27). The majority sequence is defined as the amino acid residue that appears most frequently in the group of viruses analyzed. Amino acids that are conserved across all HRV-B serotypes are in boldface, while those that vary by only one residue are underscored. -, Same amino acid as in the majority sequence.
b
See Table 2, footnote b.