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. 2004 Apr;78(7):3621–3632. doi: 10.1128/JVI.78.7.3621-3632.2004

FIG. 3.

FIG. 3.

Neurons of transgenic mice expressing BDV-N are resistant to BDV infection. (A) Transgenic (t) (+) and nontransgenic (−) Neuro-N-44 animals 3 to 5 weeks of age were infected by the intracerebral route with mouse-adapted variants of BDV strain RW98 or strain H215. At 6 weeks (H215) or 9 weeks (RW98) postinfection, mice were sacrificed and samples of brain homogenates were analyzed by SDS-PAGE and immunoblotting using a rabbit antiserum against BDV-P. (B) Immunohistochemical analysis of transgene expression and virus replication 5 weeks postinfection in the hippocampus of selected transgenic (tg+) and nontransgenic (tg−) Neuro-N-44 mice infected with H215 at the age of 4 weeks. Sagittal brain sections were stained with either MAb Bo18 (which selectively detects the transgene product) or MAb 21E7 (which detects viral P antigen in infected cells). The lower left panel shows the typical distribution of BDV-infected cells in the hippocampus of nontransgenic mice. Note that the majority of hippocampal neurons in brains of transgenic mice did not contain viral P antigen (lower right panel), indicating a lack of infection.