FIG. 5.

Mouse astrocytes can be infected with BDV. Newborn wild-type B10.BR mice were infected with BDV strain H215. At 5 weeks of age, the animals were sacrificed and sagittal sections (8 μm in thickness) of paraffin-embedded brain hemispheres were stained with a mixture of anti-P MAb 30H8 and rabbit anti-GFAP. Bound antibodies were visualized using a mixture of Cy2-labeled anti-rabbit antibody and Cy3-labeled anti-mouse antibody. An uninfected animal served as the control.