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Analysis of mRNA levels of 5 cytokines (TNF-α, IL-12 p40, IL-6, IL-15, and IL-1β) in astrocytes following infection with neurotropic viruses (MHV-A59 and MHV-JHM) and a nonneurotropic virus (MHV-2) compared with an uninfected control. Cytokine mRNA levels were examined by 24 h postinfection and were expressed in relative strength, as evaluated against the expression of a housekeeping gene, β-actin (arbitrarily designated as 1.0 U of strength).
