FIG. 4.

Coreceptor determinants for SIV entry into sMagi cells. (A) Inhibition of infection by small-molecule inhibitors. P4R5 reporter cells expressing human CD4, CCR5, and CXCR4 and sMagi cells were infected with the indicated p24/p27-normalized virus stocks in the presence or absence (Mock) of the CCR5 inhibitor TAK-779 (5 μM) or the CXCR4 inhibitor AMD3100 (1 μM). The β-galactosidase activity as a readout for infection was determined 3 days later. The results of a representative experiment are shown, and similar results were obtained in an independent experiment. (B) Inhibition of entry into sMagi cells by different concentrations of TAK-779. rhCCR5 Magi cells, which express human CD4, together with pigtailed macaque CCR5, and sMagi cells were infected as described above. Similar results were obtained in an independent experiment.