FIG. 5.

Coreceptor expression in sMagi cells and coreceptor usage of the I324L variant. (A) Analysis of coreceptor expression in sMagi cells. Total RNA was prepared from sMagi cells, DNase treated, diluted as indicated, and subjected to RT-PCR analysis with primers specific for the indicated coreceptors. (B) Coreceptor usage of the I324L variant. 293T cells were transiently cotransfected with human CD4 and the indicated coreceptors of rhesus macaque origin. The transfected cells were infected with 50 ng of the indicated luciferase reporter viruses and luciferase activity quantified. The results are shown relative to SIVmac239 entry via CD4 and CCR5 and similar results were obtained in an independent experiment.