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. 2004 Apr;78(7):3262–3270. doi: 10.1128/JVI.78.7.3262-3270.2004

FIG. 2.

FIG. 2.

Mapping of the virion secretion determinant to the AvrII-EcoRV restriction fragment. (A) Cartoon view of the constructs. Blank box, 3.4 sequence; filled box, 4B sequence. The AvrII-EcoRV restriction fragment determines the low-virion-secretion and low-HBsAg-secretion phenotypes of clone 3.4. Dimers of HBV constructs (10 μg) were transfected into Huh7 cells grown in 6-cm dishes. Cells and culture supernatant were harvested 5 days later. Shown on the right are the HBsAg and HBeAg values from 1:5-diluted culture medium, with values from nontransfected cells subtracted. (B) HBV DNA replication and virion secretion analyzed from the same experiment. Cell, intracellular HBV DNA isolated from core particles; medium, extracellular HBV DNA associated with Dane or core particles; DS, double-stranded DNA (relaxed circular or linear); SS, single-stranded DNA. For extracellular particles, DS corresponds to Dane particles while SS segregates with naked core particles (22).