Table 1.
Average Chromatographic Retention Times (tR) for Selected Flavanone and Chalcone Aglyconesa
| flavanone | tR (min) | chalcone (min) | tR (min) |
|---|---|---|---|
| 7-hydroxyflavanone | 26.3 | 2',4'-dihydroxychalcone | 32.9 |
| 3'-hydroxyflavanone | 26.2 | 3,2'-dihydroxychalcone | 32.3 |
| 4'-hydroxyflavanone | 25.7 | 4,2'-dihydroxychalcone | 32.4 |
| flavanone | 31.1 | 2'-hydroxychalcone | 36.6 |
| alpinetin | 24.9 | cardamonin | 34.0 |
a
Values were determined by averaging the retention times for the respective flavanone or chalcone in all UGT1A reactions analyzed using the Shimadzu HPLC system.