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Expression of HA-tagged mORF74. COS-7 cells were transfected with increasing amounts (1, 2, and 5 μg) of cDNA encoding HA-mORF74, 2 μg of cDNA encoding untagged mORF74, or 2 μg of cDNA encoding HA-hORF74. Forty-eight hours after transfection, vGPCR expression was determined in an ELISA. A representative experiment performed in triplicate is shown. The experiment was repeated two times.
