Skip to main content
. 2004 Apr;78(7):3419–3428. doi: 10.1128/JVI.78.7.3419-3428.2004

FIG. 5.

FIG. 5.

HIV-1 polyadenylation signal readthrough and readthrough RNA-related recombination. (A) Proviral structure, lacZ allele ratio from RNase protection assay, and blue/white ratio for vectors used to determine polyadenylation readthrough. Listed from the top downward are the wild-type lacZ vector (LacPuro), the constitutive readthrough vector (LicPuro-äc), the natural readthrough vector [LicPuro(pA)äc], and the double pA control vector [LicPuro(2pA)äc]. Arrows indicate the positions of pA signals within the vector. The RNase protection ratios of tail to body lacZ alleles and recombination rates are listed to the right. (B) RNase protection assay with viral RNAs from vectors shown in panel A. Probe, undigested probe. Signals corresponding to the protected body and tail lacZ alleles are indicated by “lic” and “-ac-,” respectively; the LacPuro body contains uninterrupted lacZ. Mock, medium from cells transfected with helper plasmid only.