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. Author manuscript; available in PMC: 2013 Nov 24.
Published in final edited form as: Science. 2013 May 24;340(6135):991–994. doi: 10.1126/science.1231041

Fig. 4.

Fig. 4

H2B-YFP expression in the nucleus as a reporter of Notch activation. (A–D) YFP fluorescence images of CHO-K1 cells show nuclear fluorescence of H2B-YFP when Notch is activated. Histograms of fluorescence intensities of single cells are also shown. (a.u.: arbitrary unit) (A) Ttol=12 pN. (B) Ttol=58 pN. (C) 12 pN TGT with DNase I digestion. (D) A control with unconjugated BSA. Here, we used BSA for TGT immobilization and surface passivation because PEG surface did not survive two days period required for our Notch reporter system.