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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Heart Rhythm. 2013 Mar 14;10(7):1001–1009. doi: 10.1016/j.hrthm.2013.03.005

Figure 3.

Figure 3

MiR-499 targets KCNN3. A: Alignment of the sequences of miR-499 with its target site in the 3′ UTRs of KCNN3. The miR-499 sequence is identical between human and mouse, and the binding site of KCNN3 is conserved among mammals. The nucleotides marked represent the miR-499 “seed site” and its paired sequence. B: Luciferase reporter assay in HL-1 cells showing co-transfection of miR-499 mimic significantly reduced luciferase activity with the 3′UTR of KCNN3 subcloned into the luciferase reporter vector. *P<0.05 vs. control, n=4 for each group. C and D: Argonaute 1 and 2 pull-down assays in HL-1 cells. The levels of KCNN3 transcripts were determined by quantitative real-time RT-PCR (C) and agarose gel electrophoresis (D).