Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: DNA Repair (Amst). 2013 May 15;12(7):518–528. doi: 10.1016/j.dnarep.2013.04.005

FIGURE 6. Effect of RECQL4 and WRN on NEIL1 incision of thymine glycol in telomeric D-Loops and forks.

FIGURE 6

Representative gels and quantitative analysis showing the effect of RECQL4 (black bars) or WRN (white bars) on NEIL1 incision activity of a thymine glycol lesion on a telomeric D-Loop DL-Tg2. 2.5 nM NEIL1 was incubated with 0.5 nM 5′-labeled substrate and increasing concentrations of RECQL4 (at molar concentrations of 1, 5, 10 and 20 against NEIL1) or WRN (at molar concentrations of 1, 2, 4 and 8 against NEIL1). Error bars show standard deviation over at least three independent assays. *P<0.05 by a t-test.