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Egr3 is required for normal neurite outgrowth and branching in NGF-treated sympathetic neurons. A, Dissociated Egr3−/− (KO) sympathetic neurons have impaired neurite outgrowth in response to NGF treatment. Neurites from Egr3 KO neurons have significantly fewer branch points (B), decreased total neurite length (C), and decreased longest neurites compared with Egr3+/+ wild-type (WT) neurons (D). *p < 0.05, N > 60 neurons of each genotype analyzed. Scale bar: A, 50 μm.
