Table 1.
qPCR-confirmed deregulated genes sampled from 30 genes with the smallest difference p value in the microarray dataset of differentially expressed genes in Egr3-deficient sympathetic neurons. (23 confirmed genes shown)
| Official gene symbol | NCBI gene ID (mouse) | Microarray |
qPCR fold change (± SEM) | |
|---|---|---|---|---|
| Difference p value | Fold change | |||
| Downregulated | ||||
| Sox9 | 20682 | 2.74 × 10−6 | −2.25 | −2.32 ± 0.08 |
| Plxnd1 | 67784 | 5.07 × 10−6 | −1.82 | −2.28 ± 0.05 |
| Esd | 13885 | 5.99 × 10−6 | −3.80 | −1.64 ± 0.12 |
| Gins2 | 272551 | 2.22 × 10−5 | −1.88 | −1.51 ± 0.07 |
| Ret | 19713 | 5.28 × 10−5 | −1.63 | −1.94 ± 0.06 |
| Insc | 233752 | 2.94 × 10−4 | −2.32 | −2.77 ± 0.48 |
| Notch1 | 18128 | 3.92 × 10−4 | −1.63 | −2.07 ± 0.30 |
| Efnb1 | 13641 | 2.75 × 10−3 | −1.40 | −2.24 ± 0.34 |
| Lmo7 | 380928 | 3.00 × 10−3 | −2.90 | −2.59 ± 0.53 |
| Hs3st2 | 195646 | 5.42 × 10−3 | −2.68 | −1.60 ± 0.25 |
| Ngfr | 18053 | 8.50 × 10−3 | −1.48 | −2.86 ± 0.19 |
| Upregulated | ||||
| B3galt6 | 117592 | 3.68 × 10−8 | 7.47 | 2.58 ± 0.11 |
| Mxra8 | 74761 | 6.73 × 10−7 | 2.20 | 1.60 ± 0.17 |
| Nf1 | 18015 | 2.05 × 10−5 | 2.87 | 2.26 ± 0.37 |
| Apbb1 | 11785 | 7.44 × 10−5 | 1.54 | 1.78 ± 0.16 |
| Pabpc1l | 381404 | 2.90 × 10−4 | 4.77 | 3.67 ± 0.23 |
| Slit2 | 20563 | 5.32 × 10−4 | 1.70 | 1.63 ± 0.20 |
| Slc15a2 | 57738 | 8.59 × 10−4 | 3.72 | 2.24 ± 0.55 |
| Irak1 | 16179 | 1.57 × 10−3 | 2.80 | 2.00 ± 0.17 |
| Zfp292 | 30046 | 1.95 × 10−3 | 2.79 | 1.50 ± 0.08 |
| Pde3a | 54611 | 6.81 × 10−3 | 3.91 | 1.97 ± 0.20 |
| Rccd1 | 269955 | 7.72 × 10−3 | 3.59 | 4.43 ± 0.45 |
| Ptpn11 | 19247 | 8.65 × 10−3 | 1.55 | 1.73 ± 0.27 |
Results represent qPCR-confirmed deregulated genes from three replicate cDNA samples (p < 0.05).