Table 2.

Selected qPCR-confirmed deregulated genes in Egr3-deficient sympathetic neurons and their GO determined functional associations

Official gene symbol	NCBI gene ID (mouse)	Microarray		qPCR fold change (±SEM)	SNS development	Axonogenesis	Dendritogenesis	Axon guidance
		Difference p value	Fold change
Downregulated
Plxnd1	67784	5.07 × 10−6	−1.82	−2.28 ± 0.05				X
Ret	19713	5.28 × 10−5	−1.63	−1.94 ± 0.06	X	X		X
Notch1	18128	3.92 × 10−4	−1.63	−2.0 ± 0.30	X	X	X
Efnb1	13641	2.75 × 10−3	−1.40	−2.24 ± 0.34		X	X	X
Ngfr	18053	8.50 × 10−3	−1.48	−2.86 ± 0.19	X	X	X	X
Upregulated
Nf1	18015	2.05 × 10−5	2.87	2.26 ± 0.37	X	X
Apbb1	11785	7.44 × 10−5	1.54	1.78 ± 0.16		X
Slit2	20563	5.32 × 10−4	1.70	1.63 ± 0.20		X	X	X
Ptpn11	19247	8.65 × 10−3	1.55	1.73 ± 0.27	X	X	X

Fold change compared to control with negative fold-change values representing genes downregulated in the absence of Egr3 and positive fold-change values representing genes upregulated in the absence of Egr3. All qPCR fold changes are significant, p < 0.05, three replicate cDNA samples.