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. Author manuscript; available in PMC: 2013 Nov 17.
Published in final edited form as: Mod Rheumatol. 2013 Jan 11;23(6):10.1007/s10165-012-0811-x. doi: 10.1007/s10165-012-0811-x

Fig 1. Induction of Th1 cells and Th17 cells from human CD4 T cells.

Fig 1

Human CD4 T cells were cultured for 5 days either under Th1 or Th17 polarizing conditions. Following FcR blocking, surface staining of CD4, and intracellular staining of IL-17 and IFN-γ, IL-17 (horizontal axis) versus IFN-γ expression (vertical axis) in the CD4(+) population was assessed by flow cytometry analysis. Representative data of three independent experiments is shown (Fig 1 upper panels). IFN-γ and IL-17 in both Th1 and Th17 polarizing cell culture supernatants were measured by ELISA (Fig 1 lower panels).