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. Author manuscript; available in PMC: 2013 Nov 17.
Published in final edited form as: Mod Rheumatol. 2013 Jan 11;23(6):10.1007/s10165-012-0811-x. doi: 10.1007/s10165-012-0811-x

Figure 2. IFN-γ, but not IL-17 induces CD40, CD54, and MHC-II on RA FLS.

Figure 2

Passage 4-8 FLS from patients with RA were cultured in CMRL culture media with 10% FCS, 1% penicillin/streptomycin, and 2% L-glutamine in the presence of either IFN-γ: 0.5 ng/ml, 5 ng/ml, or 50 ng/ml (upper panels) or IL-17: 0.1 ng/ml, 1 ng/ml, or 10 ng/ml (lower panels) for 48 hours. The FLS were stained with PE-conjugated anti-CD40, CD54, or MHC-II, followed by flow cytometry analysis. Combined results of 3 independent experiments are presented both by % expression above background or relative mean fluorescence. “FLS only” indicates FLS incubated for 48 hours without any cytokines. The data is expressed as the mean +/- standard error of the mean (** p<0.01, * p<0.05 by paired t testing compared to FLS only)