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. Author manuscript; available in PMC: 2013 Jul 14.
Published in final edited form as: J Biochem Mol Toxicol. 2011 May 2;25(5):320–329. doi: 10.1002/jbt.20392

Figure 1.

Figure 1

Drug metabolizing and antioxidant enzyme Cytochrome P450 (Cyp) 4a14, 2b10, 3a11, 2e1, NAD(P)H:quinone oxidoreductase 1 (Nqo1), Sulfotransferase (Sult) 2a1/2, Heme oxygenase-1 (Ho-1), and nuclear factor-E2-related factor-2 (Nrf2) mRNA and protein expression in livers of wild-type (WT) and hepatocyte-specific Keap1-null (KO) mice. (A). Western blot of Nrf2 proteins in nuclear fractions isolated from livers from WT and KO mice (protein loading amount 50 μg/lane, n = 4). (B) Cyp4a14, 2b10, 3a11, 2e1, Nqo1, Sult2a1/2, and Ho-1 mRNA expression. Total RNA was isolated from liver and mRNA levels were quantified using branched DNA signal amplification assay. mRNA expression level is presented as mean RLU ± SEM (n = 4 animals). Asterisks (*) represent a statistical difference between WT and KO (p < 0.05). (c). Western blots of Cyp3a11, 2e1, Nqo1, Ho-1, Gclc, and membrane fraction B-actin [β-actin(m)] and cytosolic B-actin [β-actin(c)] protein expression in liver fractions from WT and KO mice (protein loading amount 50 μg/lane, n = 4). (C). Quantification of Cyp3a11, 2e1, Nqo1, Ho-1, Gclc, and Nrf2 relative protein expressions in KO mice compared to WT. Asterisks (*) represent a statistical difference from corresponding WT (p < 0.05).