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. Author manuscript; available in PMC: 2013 Jul 14.
Published in final edited form as: J Biochem Mol Toxicol. 2011 May 2;25(5):320–329. doi: 10.1002/jbt.20392

Figure 4.

Figure 4

Multidrug resistance-associated protein 2 (Mrp2), Multidrug resistance protein 2 (Mdr2), bile salt export pump (Bsep), breast cancer resistance protein (Bcrp) mRNA and protein expression in livers of wild-type (WT) and hepatocyte-specific Keap1-null (KO) mice. (A). mRNA expression of Mrp2, Mdr2, Bsep, and Bcrp. Total RNA was isolated from liver and mRNA levels were quantified using branched DNA signal amplification assay. mRNA expression level is presented as mean RLU ± SEM (n = 4 animals). Asterisks (*) represent a statistical difference between WT and KO (p < 0.05). (B). Individual Western blots of Bcrp and Mrp2 in livers from WT and KO mice (protein loading amount 50 μg/lane, n = 4). (C). Quantification of Bcrp and Mrp2 relative protein expressions in KO mice compared to WT. Asterisks (*) represent a statistical difference from corresponding WT (p < 0.05).