Table 2.
Morphologic Abnormality at 48–52 hpf of Zebrafish Embryos Injected with WT or Mutant RNA at the 1-Cell Stage
| No Abnormalities | Heart and Facial Abnormalitiesa | Severely Disorganizedb | Total Number of Embryos | |
|---|---|---|---|---|
| WT | 125 | 7 (5.3%) | 0 (0%) | 132 |
| p.Gln79Leu | 31 | 78 (66.1%) | 9 (7.6%) | 118 |
| p.Glu81Gly | 42 | 55 (52.4%) | 8 (7.6%) | 105 |
| p.Gly95Ala | 44 | 34 (40.5%) | 6 (7.1%) | 84 |
a
Craniofacial abnormalities, pericardial heart edema, and an elongated yolk sac were observed.
b
Disorganized round body shape with a dysmorphic head and body trunk as shown in Figure S4.