Skip to main content
. Author manuscript; available in PMC: 2013 Jul 15.
Published in final edited form as: J Struct Biol. 2011 May 24;175(3):253–263. doi: 10.1016/j.jsb.2011.05.004

Fig. 4.

Fig. 4

Dynamic transfer function (DTF) and its limitation on the resolution in 3D reconstruction. (A) A diagram showing the mechanism of dynamic scattering. “D” denotes the diameter of a particle, and red spots represent atoms in the particle. Solid arrows represent the actual pathway of an electron inside the particle, and the dashed line represents the hypothetical pathway of the electron without considering the dynamic scattering. (B) Resolution limit dm imposed by ignoring dynamic scattering as a function of the particle diameter D at different accelerating voltages. (C and D) Dynamic transfer functions at the accelerating voltage of 200 kV (C) and 300 kV (D) for different particle diameters (300–1300 Å), demonstrating that a higher accelerating voltage can push the resolution limit due to dynamic scattering to a small extent.