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. 2013 Jun 25;110(28):11421–11426. doi: 10.1073/pnas.1300624110

Fig. 3.

Fig. 3.

Similar to after UV, the integrity of the replication fork DNA is maintained after DNA polymerase dissociation. The integrity of the replication fork is maintained following arrest by UV irradiation or polymerase dissociation, but collapses after disruption of the helicase. [14C]thymine-prelabeled cultures were pulse-labeled with [3H]thymidine for 20 s before being filtered and placed in nonradioactive media and either UV-irradiated with 50 J/m2 or shifted to 42 °C as indicated. The amount of radioactivity remaining in the total genomic DNA (open symbols) and nascent DNA at the replication fork (filled symbols) is plotted over time. Error bars represent SE of two independent experiments.