Fig. 6.

iRhom2 controls ADAM17-dependent keratinocyte migration. (A and B) Primary WT (A) or iRhom2^−/− (B) keratinocytes from 12-wk-old animals were cultured to confluence, and then a scratch wound was introduced, and the cultures treated with or without FGF7 (50 ng/mL) or HB-EGF (50 ng/mL), as indicated. Micrographs were taken at 0 and 48 h after scratch wounding. (Scale bar: 100 μm.) (C and D) Quantification of the results obtained with WT keratinocytes (C) or iRhom2^−/− keratinocytes (D) (n = 3). (E) Western blot of ERK1/2 phosphorylation in primary WT or iRhom2^−/− keratinocytes incubated with or without FGF7 (20 ng/mL) or HB-EGF (50 ng/mL) (ERK1/2 was loading control in E). (F) Densitometric quantification of the levels of pERK1/2 of three experiments like the one shown in E. *P ≤ 0.05; ±SEM.