Fig. 4.

FUS3 deletion alters normal cell cycle progression and affects cell-cycle modulation of the pheromone signaling: (A) Distributions of WT (Top) and fus3Δ (Bottom) cell-cycle periods of young daughters (gray) and mothers (black) under normal conditions and of all cells (combined mother and young daughter cells) under 1 h stimulation of 100-nM αF (blue). Only cell-cycle periods that started before stimulation were measured. (B) Median response, plotted as percentage of maximum response, and median cell-cycle period (C) of WT and fus3Δ cells as a function of their position through the cell cycle at the time of stimulation (measured as percent of the progression through the cell-cycle period). The beginning of the cell cycle was defined as the time point when the image algorithm recognizes a new bud, corresponding approximately to G2 phase. Estimated cell-cycle phases are depicted in the plot.