Fig. 3.

TF variant-induced proliferation is integrin dependent. (A) pcDNA control cells or asTF-expressing 2A3-3 cells were transduced with shRNA to silence β1 integrins, and proliferation of these cells was compared with control after 3 d. (B) 2A3-3-pcDNA or -asTF cells were mock-treated or incubated with a β1 (epitope 579–799)-reactive antibody. The cells were lysed in Brij78-containing lysis buffer and β1 integrin was precipitated using AIIB2. asTF coimmunoprecipitation was assessed by Western Blot. (C) 2A3-3-pcDNA cells were preincubated with 10 nM recombinant asTF in the presence or absence of an antibody against β1 integrin epitope 579–799 and seeded on collagen I. After washing, remaining cells were counted. (D) 2A3-3-asTF cells or 2A3-3-pcDNA cells, untreated or incubated with 10 nM recombinant asTF, were fixed and stained with AIIB2 (total β1 integrin; green) and HUTS-21 (activated β1 integrin; red). (Scale bars, 50 μm.) *P < 0.05 and **P = 0.01.