Fig. 2.

Interferon-γ (IFN-γ) production from splenocytes and cervical lymph node cells. Each group of vaccinated mice (n = 5) was sacrificed at day 10. The splenocytes (A) and cervical lymph node cells (B) were prepared to determine IFN-γ production. Immune cells were stimulated in vitro with the E6/E7 peptides (1 µg/mL each) for 3 days. The number of E6/E7 peptide-specific IFN-γ producing cells generated in vaccinated mice was determined by group by ELISPOT assay. The Student's t-test was used to compare the IFN-γ production between two groups. ^*p < 0.05 and ^**p < 0.01.