Fig. 1.

The effects of RASSF1A on cell morphology and migration. (A) H1299 cells stably transfected with RASSF1A or pcDNA₃. RASSF1A expression was analyzed by reverse transcription polymerase chain reaction (RT-PCR) and western bolt analysis. GAPDH and β-actin served as a loading control. (B) RASSF1A stable transfected H1299 cells and control cells were photographed before the use for the migration assay at 20× magnification. (C) The results of wound healing assay. Cells were grown to confluence, and the wound was made by scraping the cell monolayer with a pipette tip and left for 24 to 72 hours before photographing (D) the results of transwell assay. Cells were plated at 2×10⁴ cells per upper chamber together, along with serum free medium and 10% fetal bovine serum placed in the low chamber. The cells were then allowed to migrate for 48 hours. The membrane inserts were stained with crystal violet stain and photographed at 10× magnification. Quantification of relative migration of RASSF1A stably transected H1299 cells in relation to vector control cells (p<0.05).