Fig. 3.

The effects of RASSF1A on cellular localization of histon deacetylase 6 (HDAC6) and acetylated α-tubulin (Ac α-tubulin) protein. (A) The results of co-localization of acetylated α-tubulin and HDAC6 protein. Double-immunostaining with an anti-acetylated α-tubulin antibody (green) and anti-HDAC6 antibody (red) in RASSF1A-transfected H1299 cells and control H1299 cells. (B) Co-localization of RASSF1A with HDAC6 protein in cytoplasm. H1299 cells were transiently transfected with expression vectors carrying GFP-alone or GFP-tagged RASSF1A and immunostained with an anti-HDAC6 primary antibody followed by red fluorescence dye conjugated anti-rabbit secondary antibody. (C) The results on cytoplasmic and nuclear extract from RASSF1A-transfected H1299 cells and control cells. Cells were prepared and the proteins indicated were analyzed by western blot analysis.