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. 2009 Jun 26;137(7):1235–1246. doi: 10.1016/j.cell.2009.04.024

Figure 2.

Figure 2

Engineering an Interstitial Duplication on Chromosome 7

(A) Insertional double targeting and genomic coordinates, NCBI build m37. S, SacI; T, Tth111I.

(B) Southern blot analysis of ES cell DNA samples.

(C) Cre/loxP recombination generates duplication and deletion chromosomes.

(D) Southern blot analysis of ES cell DNA confirming the duplication.

(E) Confirmation by FISH. The probes used are shown on the left. The red and green bars represent the probes located within and outside the duplicated region, respectively. The white arrow and arrowhead represent the Dp and Df alleles, respectively.

(F) A BAC array-CGH profile of chromosome 7 from mice with the duplication. Log2-transformed hybridization ratios of duplicated mouse DNA versus WT DNA are plotted.