FIG. 2.

GFP-GR exchange at the array is an energy-dependent process. A 30-min treatment with 10 mM sodium azide and deoxyglucose leads to a marked reduction in the exchange rate at the array (a) and elsewhere in the nucleus (b). Transferring the cells to a glucose-free medium (deoxyglucose only) for 60 to 90 min induces an ∼5% immobile fraction at the array (c), but the exchange at other sites in the nucleus does not show an immobile fraction (d). (e) Deoxyglucose washout eliminates the immobile fraction at the array.