FIG. 1.

Fusion protein induction and microarray analyses. (a) Western blot analysis of PML-RARα and PLZF-RARα expression with anti-RARα antibodies. Expression of AML1-ETO in transfected U937 cells was demonstrated by Western blotting with anti-ETO antibodies. Similar amounts of proteins (10 μg) of the same protein lysates were used in both experiments. The reason for the lower expression of RARα in the U937-control cells is unclear. (b) Significance Analysis of Microarray softwared was used as a paired analysis to identify genes that were differentially regulated between fusion protein-positive (U937-AML1-ETO, U937-PML-RARα, and U937-PLZF-RARα) and fusion protein-negative cells (U937-vector control plus zinc and U937-PML-RARα without zinc). The graph indicates the expected as well as the observed variability. Induced and repressed genes are indicated in red and green, respectively.